The UV andor visible spectrophotometer is a widely used analytical tool based on a solution’s ability to absorb light. This classic instrument is used for both qualitative and quantitative determinations from molecular identification and assays to colorimetric determination of specific ions. Procedures for using spectrophotometers have been developed in response to inspections by both quality assurance departments and by regulatory bodies. Proof of instrument validation must be available to regulatory bodies upon request; therefore laboratories need to make sure that the instrument is within specification.

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